The Journal of clinical endocrinology and metabolism
Authors: Veldhuis-Vlug AG, Woods GN, Sigurdsson S, Ewing SK, Le PT, Hue TF, Vittinghoff E, Xu K, Gudnason V, Sigurdsson G, Kado DM, Eiriksdottir G, Harris T, Schafer AL, Li X, Zaidi M, Rosen CJ, Schwartz AV
Journal of hospital medicine
Authors: Block BL, Martin TM, Boscardin WJ, Covinsky KE, Mourad M, Hu LL, Smith AK
Journal of the American Geriatrics Society
Authors: Gerlach LB, Kales HC, Kim HM, Zhang L, Strominger J, Covinsky K, Teno J, Bynum JPW, Maust DT
Global advances in health and medicine
Authors: Purcell N, Zamora K, Bertenthal D, Abadjian L, Tighe J, Seal KH
TO REMOVE
Authors: Boden M, Zimmerman L, Azevedo KJ, Ruzek JI, Gala S, Abdel Magid HS, Cohen N, Walser R, Mahtani ND, Hoggatt KJ, McLean CP
TO REMOVE
Authors: An P, Sezgin E, Kirk GD, Duggal P, Binns-Roemer E, Nelson G, Limou S, Van Natta ML, Jabs DA, Estrella M, Kopp JB, Winkler CA
The American journal of medicine
Authors: Packer CD, Duca NS, Dhaliwal G, Ismail N, Pincavage AT, Kisielewski M, DeWaay D, Walsh K, Lai CJ
Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA
Authors: Barzilay JI, Buzkova P, Djoussé L, Ix J, Kizer J, Cauley J, Matthan N, Lichtenstein AH, Mukamal KJ
Journal of virological methods | Volume 292
Authors: Telwatte S, Kumar N, Vallejo-Gracia A, Kumar GR, Lu CM, Ott M, Wong JK, Yukl SA
A hallmark of coronavirus transcription is the generation of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and an array of subgenomic mRNAs (sgRNAs) encompassing sequences arising from discontinuous transcription. Existing PCR-based diagnostic assays for SAR-CoV-2 are qualitative or semi-quantitative and do not provide the resolution needed to assess the complex transcription dynamics of SARS-CoV-2 over the course of infection. We developed and validated a novel panel of sensitive, quantitative RT-ddPCR assays designed to target regions spanning the genome of SARS-CoV-2. Our assays target untranslated regions (5', 3') as well as different coding regions, including non-structural genes that are only found in full length (genomic) RNA and structural genes that are found in genomic as well as different subgenomic RNAs. Application of these assays to clinically relevant samples will enhance our understanding of SARS-CoV-2 gene expression and may also inform the development of improved diagnostic tools and therapeutics.
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eLife
Authors: Cauley JA, Shoback DM
